摘要
[目的]筛选出拮抗致病大豆疫霉的真菌菌株，为深入研发生物菌剂提供依据。[方法]采用平板对峙法测定40株野生大豆真菌对疫霉菌的抑制作用。[结果]供试的40株菌株，不同菌株间菌落型态差异较大。从光滑程度分析，36份菌落表面不光滑，3份表面光滑并显浅黄色；从菌落颜色分析，正面以白色为主，其次是黄色和粉色；反面则以紫色为主，其次为黑色和绿色；从菌落生长型态分析，大多数菌落呈白色絮状突起或羊毛状菌丝；11株野生大豆内生真菌的抑菌率变化幅度为62％～81％，80％以上共1株Y4－S（Ⅰ），75％～80％共2株，70％～75％共3株，其余5株均低于70％。抑菌率从大到小依次为Y4－S（Ⅰ）、Y5－r（Ⅱ）－1、Y6R－15－1、Y1－r－3和Y5R4、Y6S－26、Y5LB、Y5r－12、Y2－5（Ⅱ）、Y2－S、Y5R18－2－1。[结论]最强拮抗菌株Y4－S（Ⅰ）、Y5－r（Ⅱ）－1、Y6R－15－1显微型态相似，呈枝状，有隔，初步判断可能来源同一属，具体属种需要进一步判断。
Abstract
Objective] Screening of antagonistic phytophthora pathogenic fungal strains provide the basis for further research and development of biological agents.[Method] Forty strains of fungus were tested.The inhibitory effect of wild soybean fungi on the wild soybean was deter-mined by the method of plate confrontation.[ Result] Among the tested 40 strains colony morphology are quite different from each other.On the analysis of smoothness, there are thirty-six colonies whose surface is not smooth, while three colonies appear smooth and pale yellow; On the analysis of colony colors, the front side is mainly white, followed by yellow and pink while, and the back side is mainly purple, followed by black and green;On the analysis of colony growth pattern, most of the colonies appear white flocculent protrusions or wool like hyphae.The inhibitory rates of 11 strains of wild soybean endophytic fungus are 62%-81%, with one strain of Y4-S ( I) more than 80%, two strains in 75%-80%, three strains in 70%-75%, and the remaining five strains are less than 70%.Inhibition rate in descending order is Y4-S(Ⅰ) , Y5-r(Ⅱ)-1,Y6R-15-1,Y1-r-3,Y5R4, Y6S-26, Y5LB, Y5r-12, Y2-5(Ⅱ),Y2-S,Y5R18-2-1.[Conclusion] The strongest antagonistic effect of strains are Y4-S (Ⅰ), Y5-r(Ⅱ)-1 and Y6R-15-1.They have similar microscopic form as dendritic, and they have separated.After a preliminary judgment, they may be a genus of unity, but their specific species require further judgment.
基金项目
黑龙江省自然科学基金( C2015008)
黑龙江省哈尔滨市人才专项(2015RQQXJ019)
国家青年基金(31101171)
东北农业大学创新项目SPIT(201610224154)
万方数据