摘要
[目的]以鲣鱼骨为原料，对鱼骨硫酸软骨素提取方法进行研究。[方法]以硫酸软骨素提取率为评价指标，通过单因素和正交试验优化鲣鱼骨硫酸软骨素的提取条件。[结果]在酶添加量2．5％（木瓜蛋白酶：胰蛋白酶为1∶2）、料液比1∶20、提取温度80℃、pH 6．5下浸提24 h、超声处理30 min，鱼骨硫酸软骨素的提取率达2．86％，纯度为90．0％。理化分析显示，鲣鱼骨硫酸软骨素颜色洁白，水分含量9．2％，氨基己糖含量30．1％，氮含量3．05％，葡萄糖醛酸含量26．9％。通过红外光谱分析，确定样品为硫酸软骨素C。[结论]利用优化工艺提取鲣鱼骨中的硫酸软骨素具有可行性，在鲣鱼加工副产物的高值化利用中具有一定的应用前景。
Abstract
Objective] To research the extraction methods for chondroitin sulfate (CS) with Katsuwonus pelam is bones as the raw materials. [ Method] With the extraction rate of chondroitin sulfate as the evaluation index,the extraction condition for chondroitin sulfate was optimized by single factor test and orthogonal test.[Result] Under the conditions of 2.5%enzyme supplementation (papaini∶trypsin=1∶2),1∶20 solid-liquid ratio,80 ℃ extraction temperature,pH 6.5,extraction for 24 h,and ultrasonic treatment for 30 min,the extraction rate of chondroitin sulfate reached 2.86% and the purity was 90.0%.Physical and chemical analysis showed that chondroitin sulfate was white,containing 9.2%moisture, 30.1% hexosamine,3.05% nitrogen,and 26.9%glucuronic acid.Infrared spectroscopic analysis showed that the sample was chondroitin sulfate C.[Conclusion] It is feasible to extract chondroitin sulfate from Katsuwonus pelamis bones by the optimized method,showing some potential for value-add utilization in Katsuwonus pelamis processing wastes.
国家科技期刊平台
NSTL
维普
万方数据