摘要
[目的]研究三孢布拉霉菌发酵生产β－胡萝卜素的代谢调控途径，为通过优化发酵培养条件来提高β－胡萝卜素的产量提供依据和参考。[方法]采用紫外分光光度计测定β－胡萝卜素的含量，优化正负菌孢子接入种子培养基的接种比例，通过对三孢布拉霉菌发酵生产β－胡萝卜素的代谢调控分析，确定最佳补液时间，优化培养温度和接种比例来提高β－胡萝卜素的产量。[结果]最佳孢子接种比例为正菌∶负菌＝10∶1，最佳补液时间为52 h，最佳培养温度为27℃，最佳种子液接种比例为8％。[结论]通过对菌体生长代谢的分析优化了培养条件，并将β－胡萝卜素的产量从1．45 g／L提高到2．26 g／L，产量提高了55．9％，取得了较好的效果，为β－胡萝卜素的工业化生产奠定了基础。
Abstract
Objective] The aim was to study the metabolic control of fermentative production of β-carotene by Blakeslea trispora,which provide a basis for increasing the production ofβ-carotene by optimized conditions.[Method] Through determiningβ-carotene content by ultraviolet spec-trophotometer,the inoculation ratio of plus( +) and minus(-) spores were optimized.Through study on the metabolic control of efficient synthesis of β-carotene by mated Blakeslea trispora,the optimal medium supply time,culture temperature and inoculation amount was determined.[ Result] After above,the optimum conditions were obtained,with the inoculation ratio of plus(+) and minus(-) spores was 10∶1,the time of medium sup-ply was 52 hours,the culture temperature was 27℃,the inoculation amount was 8%.[ Conclusion] Through study on the metabolic control of fer-mentation production ofβ-carotene by Blakeslea trispora, the optimum conditions were obtained.As a result,the production ofβ-carotene was in-creased from 1.45 to 2.26 g/L,the rate of increase was up to 55.9%,which lays a foundation for the industrial production of β-carotene.
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