摘要
[目的]优化普洱茶中茶多酚的提取工艺条件。[方法]以购自云南省临沧市的普洱茶为原料,以总多酚提取率为优化目标,在单因素试验基础上,通过响应面分析法分析乙醇浓度、浸提时间、浸提温度和料液比对普洱总茶多酚提取的影响。[结果]确认普洱茶中茶多酚提取最佳工艺为浸提时间1 h、浸提温度90℃、料液比1∶25 g/mL、乙醇浓度75%。实际测得的茶多酚得率为17.0487%,和模型预测值相符。[结论]研究可为普洱茶中茶多酚的工业化提取生产提供参考依据。
Abstract
Objective] The aim was to optimize conditions for extracting polyphenols from Pu’er tea.[Method] With Pu’er tea from Lincang City,Yunnan Province as raw materials, total polyphenol extraction rate as the optimization objective,on the basis of single factor experiment, effects of ethanol concentration,extraction time,extraction temperature and ratio of material to liquid on extraction of total polyphenols from Pu’ er tea were analyzed by response surface analysis.[ Result] The optimal conditions were as following:extraction time 1 h,extraction temperature 90℃,solid-liquid ratio 1∶25 g/mL,ethanol concentration 75%.The actual yield of polyphenol was 17.048 7%,which was in agreement with the predicted values.[Conclusion] The study can provide reference basis for industrial extraction and production of polyphenol in Pu’er tea.