摘要
[目的]研究环境因子对马铃薯致病立枯丝核菌AG－3融合群生长状况的影响，以明确黑龙江省马铃薯立枯丝核菌的环境适应性，为该病害的防治提供理论依据。[方法]采用生长速率法对立枯丝核菌菌丝在不同温度、pH、光暗条件下的生长状况进行测定。[结果]菌丝在4℃条件下不能生长，适宜温度为19～25℃，全暗和全光条件下最适生长温度分别为22和25℃，黑暗条件有利于菌丝生长；在pH 4～11时菌丝均能生长，以pH 6～7时菌丝生长速度较快；当热激20 min以上时，其致死温度为50℃。[结论]综合评价了环境因子对立枯丝核菌AG－3融合群生长的影响，同时耐热性测定表明短暂热激对菌丝生长有一定的促进作用，但当时间过长或温度过高时会严重抑制菌丝的生长。
Abstract
Objective] The aim was to study effects of environmental factors on the growth status of anastomosis group AG-3, to determine envi-ronmental adaptation of Rhizoctonia solani from Heilongjiang Province, which provides a theoretical basis for control of the disease.[ Method] The growth status of Rhizoctonia solani under different conditions of temperature, pH and light was determined by growth rate method.[ Result] The mycelium could grow under the setting temperature except 4 ℃, the optimal temperature was 19-25 ℃, and the fastest growth under the condition of dark and light was 22 and 25 ℃ respectively, and the growth of mycelia was accelerated by darkness.The range of pH was 4-11, and the optimal pH for growth was 6-7.Meanwhile, the lethal temperature was 50 ℃ after heat shocking more than 20 minutes.[ Conclusion] The effect of environmental factors on the mycelial growth of AG-3 is comprehensively evaluated, and determination of heat resistance indicates that there is a promotion on mycelial growth by heat shocking when the time and temperature is not too long or too high.
基金项目
科技部“十二五”农村领域国家科技计划研究项目(2012BAD06B02)
国际合作项目(2013DFA31970)
黑龙江省农科院“引进博士人才科研启动金”项目(201507－15)
国家科技期刊平台
NSTL
维普
万方数据