摘要
[目的]通过优化CTAB提取法从少量的野山参须根提取DNA，使其保持野山参形态的完整。[方法]综合比较CTAB提取法及其他3种DNA提取法，筛选出较为适合少量药材DNA的提取方法。[结果]从5 mg人参根须提取DNA，栽培参DNA浓度为200～1000 ng／μL，野山参为50～100 ng／μL，电泳可检测到清晰的条带，满足浓度和纯度的要求。[结论]该研究采用改进的高盐SDS法能从5 mg微量人参须根材料中提取高质量DNA，满足浓度和纯度的要求，可作为其他药材微量组织提取DNA 的参考方法。
Abstract
Objective] The aim was to optimize CTAB method for DNA extraction from a small amount of fibrous roots of wild ginseng to keep the integrity form of wild ginseng.[Method] CTAB extraction method and other three kinds of DNA extraction method were comprehensively compared, CTAB method which is suitable for DNA extraction from a small amount of medicinal herbs was selected.[Result] DNA was extrac-ted from 5 mg ginseng root, the concentration of cultivated ginseng DNA was 200-1 000 ng/μL, wild ginseng was 50-100 ng/μL, electropho-resis can detect clear strip, meet the requirements of the concentration and purity.[ Conclusion] The improved high salt SDS method can extract high quality DNA from 5 mg trace ginseng fibril material.This method meet the requirements of the concentration and purity, and can be the ref-erence method for extracting DNA from other medicinal materials.
国家科技期刊平台
NSTL
维普
万方数据