两步培养法测定厚垣孢普可尼亚菌产孢的最佳条件
Optimum Conditions for Sporulation of Pochonia chlamydosporia in Two-stage Cultivation
石妍 1肖顺 1刘国坤 1张绍升1
作者信息
- 1. 福建农林大学植物保护学院，福建福州350002
- 折叠
摘要
[目的]探讨厚垣孢普可尼亚菌产厚垣孢子的条件。[方法]采用两步培养法测定碳源、氮源、碳氮摩尔比、pH等不同营养环境条件对厚垣孢普可尼亚菌PC021120－152菌株产厚垣孢子的影响。[结果] PC021120－152菌株的最佳产厚垣孢子条件为：初始碳源浓度为3．00 g／L，碳氮摩尔比为2．5∶1，最佳碳氮源组合为蔗糖／NaNO3，培养基最佳pH为8．0。 PC021120－152菌株的最佳产分生孢子条件为：初始碳源浓度为3．00 g／L，碳氮摩尔比为2．5∶1，最佳碳氮源组合为D－果糖／NaNO3，最佳pH为5．0。[结论]该研究结果可为厚垣孢普可尼亚菌生防制剂的生产调控提供依据。
Abstract
Objective] To discuss the nutritional conditions for sporulation of Pochonia chlamydosporia.[Method] A two-stage cultivation meth-od was used to determine the effects of various nutritional and environmental conditions ( carbon sources,nitrogen sources,carbon-nitrogen molar ratio,and pH) on the sporulation of P.chlamydosporia PC021120-152.[Result] Chlamydospore production of PC021120-152 strains was the high-est when the initial carbon concentration was 3.00 g/L;the carbon-nitrogen molar ratio was 2.5∶1;sucrose/NaNO3 was as the carbon/nitrogen source,and the pH was 8.Conidiophore production of PC021120-152 strains was the highest when the initial carbon concentration was 3.00 g/L;the carbon-nitrogen molar ratio was 2.5∶1;D-fructose/NaNO3 was as the carbon/nitrogen source,and the pH was 5.0.[Conclusion] The re-search provides references for industrialization of biocontrol agents of P.chlamydosporia.
关键词
厚垣孢普可尼亚菌/产孢/营养条件Key words
Pochonia chlamydosporia/Sporulation/Nutritional conditions引用本文复制引用
基金项目
中国烟草总公司福建公司科技项目(闽烟2012［041］号)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据