摘要
[目的]优化台蘑小香蕈液体发酵培养基的最佳配方。[方法]通过单因素试验考察葡萄糖、蔗糖、马铃薯汁、无机盐等常用碳源与氮源对台蘑液体发酵菌丝生物量的影响。[结果]台蘑小香蕈液体发酵培养基的最佳配方为蔗糖3.0%、麸皮汁3.0%、玉米粉5.0%、马铃薯汁30%、植被汁10%、NaNO30.3%、K2 HPO40.1%、KCl 0.1%、FeSO40.001%,pH 7。[结论]优化后的培养基配方能明显提高台蘑液体发酵的菌丝生物量,配方经济合理。
Abstract
Objective] To optimize the best formula of liquid fermentation mediums of small mushrooms from Wutai Mountains.[Method] Through a single factor experiment, the effects of commonly used carbon and nitrogen sources ( glucose, sucrose, potato juice, and inorganic salt) on the mycelial biomass of small mushrooms from Wutai Mountains were studied .[ Result] The best formula of the liquid fermentation medium of small mushrooms from Wutai Mountains are shown as follows: sucrose3 .0%, bran juice 3.0%, corn flour 5.0%, potato juice 30%, vegetation juice 10%, NaNO3 0.3%, K2HPO4 0.1%, KCl 0.1%, FeSO4 0.001%, and pH=7.[Conclusion] The optimized formula of the liquid fermentation medium can significantly improve the mycelial biomass of small mushrooms from Wutai Mountains , so it is economi-cal and reasonable.