摘要
[目的]针对枣裂果问题，探讨枣果纤维素酶对裂果发生的影响。[方法]以陕北主栽品种木枣、骏枣和团枣的不同成熟期枣果的果皮和果肉为材料，采用还原糖法（ DNS法），通过酶液提取和酶活性测定，分析不同样品中的纤维素酶活性，探讨不同裂果性品种、不同时期以及枣果不同组织中纤维素酶活性的动态变化趋势。[结果]抗裂性木枣不同成熟期纤维素酶活性变化差异显著，其中脆熟期活性最高；极易裂性骏枣不同时期酶活性变化也呈显著差异，裂果后活性明显下降；较易裂性团枣不同时期酶活性变化差异不显著。不同组织间纤维素酶活性变化趋势基本相同，差异均不显著。不同品种间易裂性骏枣纤维素酶活性高于抗裂性木枣和较易裂性团枣。[结论]易发生裂果的脆熟期酶活性高，抗裂性好，而裂果一旦发生会导致酶活性降低，并且裂果现象的发生与组织间的酶活性变化没有关系。
Abstract
Objective] Aiming at the problem of jujube fruit cracking,the influence of cellulase on jujube fruit cracking was discussed.[Meth-od] With the main varieties of jujube in Northern Shaanxi,Mu jujube, Jun jujube and Tuan jujube,in different fruit maturity stage and different tissue as materials,by the method of DNS,through the extraction of enzyme,the measurement of enzyme activity,enzyme activity of cellulose in different samples were analyzed,the dynamic change trend of cellulase activity in different fruit varieties,periods and tissues were discussed.[ Re-sult] The changes of cellulose activity in different mature period was significant in crack resistance jujube,which the activity of brittle ripe stage was highest;The enzyme activity changes in different periods of easy to crack of Jun jujube also showed significant difference,activity decreased significantly after cracking;The enzyme activity in different periods of easier to crack of tuan jujube was not significant.The trend of cellulase ac-tivity in different tissues was basically the same,and the difference was not significant.Among different varieties,the cellulase activity of fissility Jun jujube were higher than that of the crack resistance of the Mu jujube and Tuan jujube fissility group.[Conclusion] The enzyme activity in brittle ripe stage is highest,crack resistance is good,and cracking event will lead to reduction of enzyme activity,and there is no relationship be-tween fruit cracking and changes of enzyme activities in tissues.
基金项目
陕西省教育厅专项科研计划项目(2013 JK0735)
延安市科学技术研究发展计划项目(2013－KN12)
延安大学校级科研计划项目(YDK2015－76)
陕西省科技厅农业科技创新与攻关项目(2016NY－138)
延安市科学技术研究发展计划项目(2014CGZH－09)
国家科技期刊平台
NSTL
维普
万方数据