摘要
[目的]研究金针菇、平菇多糖体外复配的抗氧化活性，以羟基自由基（OH?）和超氧阴离子（O2－?）为评价指标，探究多糖复配的可行性和有效性。[方法]以金针菇、平菇为原材料，优化多糖提取条件，然后进行体外抗氧化活性研究，测定其对OH?和O2－?清除率，研究复合多糖对自由基的清除能力，并与单一多糖进行比较。[结果]多糖的最优提取条件为超声时间30 min，热水浸提温度为90℃。在此条件下，金针菇得率为1．25％，多糖纯度为84．3％；平菇得率为1．46％，多糖纯度76．4％。复合多糖清除OH?的能力随着浓度的升高而升高，在金针菇多糖∶平菇多糖＝5∶1的复配比例下会体现良好的协同性，优于单一多糖。复合多糖清除O2－?的能力也随着浓度的升高而升高，尤其在低浓度下，金针菇多糖∶平菇多糖＝3∶1复配时具有比单一多糖更好的协同性。[结论]食用菌多糖的复配能有效提高其抗氧化活性。
Abstract
Objective] To study the antioxidant activity of polysaccharides of Flammulina velutipes and Pleurotus ostreatus compound in vitro,to explore the feasibility and effectiveness of compound polysaccharides with hydroxyl radical ( OH? ) and superoxide anion ( O2-? ) as evaluation indexes.[Method] With F.velutipes and P.ostreatus as the raw materials,extraction condition of polysaccharides was optimized.Antioxidant activ-ity in vitro was researched to measure the clearance rates of OH? and O2-? ,to study the scavenging activity of free radical of compound polysac-charides,and to compare it with single polysaccharide.[Result] The optimal extraction conditions of polysaccharide were 30 min ultrasonic wave, 90 ℃ extraction temperature of hot water.Under this condition,the extraction rate of F.velutipes was 1.25%and the purity of polysaccharides was 84.3%.The extraction rate of P.ostreatus was 1.46%,and the purity of polysaccharides was 76.4%.The ability of compound polysaccharides to remove OH? rose to higher concentration.Under F.velutipes∶P.ostreatus =5∶1,compound polysaccharides reflected good cooperativity,which was superior to single polysaccharide.The ability of compound polysaccharides to remove O2-enhanced in higher concentration.Especially under low concentration and F.velutipes∶P.ostreatus=3∶1,compound polysaccharides showed better cooperativity.[Conclusion] The compound of edi-ble fungus polysaccharides can effectively improve its antioxidant activity.
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