摘要
从疑似感染的成年散养鸡蛋壳和发病雏鸡的肝脏、脾脏中分离到2株细菌，通过培养特性、镜检结果、生化鉴定、血清学分型等，2株分离细菌均被鉴定为鼠伤寒沙门氏菌，分别命名为AH－DY01和AH－DY02。药物敏感性检测结果表明，AH－DY01对氟苯尼考、左氧氟沙星和环丙沙星敏感，对其他12种抗生素耐药；AH－DY02对阿米卡星、氟苯尼考、左氧氟沙星、复方新诺明、庆大霉素、链霉素、环丙沙星和大观霉素敏感，对其他7种药物耐药，2株沙门氏菌均表现多重耐药性。动物回归试验结果表明，2株鼠伤寒沙门氏菌均可致死雏鸡。对分离菌的16S rDNA进行测序与遗传进化分析，结果表明AH－DY01与标准菌株ATCC－1592和ATCC13311的同源性最高，而AH－DY02与日本分离株之间的亲缘关系最近。
Abstract
Two bacterial strains were isolated from the eggshells of free-range hens and the liver and spleen of diseased chicks.Salmonella ty-phimurium strains AH-DY01 and AH-DY02 were identified according to culture characteristics, microscopic examination, biochemical charac-teristics, serological typing and so on.Drug sensitive test showed that the AH-DY01 was sensitive to florfenicol, levofloxacin and ciprofloxa-cin, and was resistant to other 12 antibiotics.AH-DY02 was sensitive to amikacin, florfenicol, levofloxacin, ciprofloxacin, Trimethoprim-Sul-famethoxazole( TMP-SMZ) , gentamicin, streptomycin and spectinomycin, and was resistant to other 7 antibiotics.Two strains of Salmonella typhimurium showed multi-drug resistant.Animal experimental results showed that the two strains both could cause chicken death.16S rDNA sequencing and phylogenetic analysis showed that homology of AH-DY01 with standard strain ATCC-1592 and ATCC 13311 was the highest, while there was the highest homology between AH-DY02 and Japan isolated strains.
基金项目
安徽省农业科学院院长青年创新基金(15B0414)
国家现代农业产业技术体系项目(CARS－41)
安徽省农业科学院科技创新团队项目(11C0404)
国家科技期刊平台
NSTL
万方数据