摘要
[目的]建立治疗奶牛胎衣不下的中兽药宫衣净酊的质量标准。[方法]采用薄层色谱(TLC)法对该酊剂中的中药材进行定性鉴别,确定该酊剂中葛根、红花、急性子和川牛膝的薄层鉴别方法。[结果]在选定的色谱条件下,色谱斑点分离较好,该鉴别方法能够有效鉴别葛根、红花和急性子。[结论]该试验中所建立的TLC方法简单、易操作、专一性强、重复性好,可为宫衣酊剂的质量控制提供依据。
Abstract
Objective] To establish quality standards of Gongyijing tincture to cure retained fetal membranes.[Method] Thin layer chroma-tography ( TLC) method was used for qualitative identification of the traditional Chinese medicine in Gongyijing tincture.Puerarin, safflower and garden balsam seed and Radix Cyathulae were identified by TLC in Gongyijing tincture.[ Result] Under the selected chromatographic conditions, chromatographic spots were better separated.This identification method could effectively identify puerarin, safflower, and garden balsam seed.[Conclusion] The established TLC method was simple, easy to operate, has strong specificity and good repeatability, which pro-vides references for the quality control of Gongyijing tincture.
基金项目
公益性行业(农业)科研专项(201303040)
中国农业科学院中兽医与临床创新团队项目(CAAS -ASTIP -2015-LIHPS-06)