摘要
[目的]研究苗期不同抗性大豆荧光参数和叶绿素含量对大豆花叶病毒（SMV）胁迫的响应。[方法]以感SMV品种合丰25和抗SMV品系牡304为材料，以东北地区SMVⅠ号株系为毒源，测定接种SMV后苗期不同抗性大豆荧光参数及叶绿素含量的变化。[结果]与对照相比，SMV胁迫下，除Fo 和qN升高外，其他荧光参数均有所降低，抗SMV品种（系）的变化幅度较小。随着胁迫时间的推移，Fm、Fm ’、ΦPSI和Fv／Fm 逐渐降低，Fo 和qN逐渐升高，qP先降低后升高，且抗SMV品系牡304和感SMV品种合丰25分别在6 d和12 d出现最低值。受SMV胁迫后，叶绿素a和叶绿素b含量的变化趋势相同，抗病品种（系）叶绿素含量的上升幅度大于感病品种（系），感病品种（系）叶绿素含量的降低率大于抗病品种（系）。[结论]苗期荧光参数以及叶绿素含量可作为鉴定大豆对SMV抗性的重要依据。
Abstract
Objective]The aim was to study effect of SMV stress on chlorophyll fluorescence parameters and chlorophyll content of soybean with different resistant levels at seedling stage.[Method] Hefeng25 and Mu304 were used as experiment materials,and virus source was SMVⅠ,effect of SMV stress on chlorophyll fluorescence parameters and chlorophyll content of soybean with different resistant levels at seedling stage was stud-ied.[Result] The result showed that compared with control,under SMV stress,fluorescence parameters declined except Fo and qN increasing,and change range of SMV-resistant variety(line) was smaller.Fm,Fm’,ΦPSI and Fv/Fm declined,Fo and qN increased and qP firstly decreased and then increased with the day passing,the minimum qP value of Mu304 and Hefeng 25 respectively happened in sixth and twelfth day.Changes of chlorophyll a and b were same under SMV stress,change range of chlorophyll content resistant variety( line)>susceptible variety( line) ,reduction rate of chlorophyll content resistant variety(line)>susceptible variety(line).[Conclusion] Chlorophyll fluorescence parameters and chlorophyll content at seedling stage can be used as important basis for identifying SMV resistance of soybean.
基金项目
国家自然科学基金面上项目(31371651)
中国科学院大豆分子设计育种重点实验室开放课题(SMDB-02)
国家科技期刊平台
NSTL
维普
万方数据