福林试剂法快速检测藤仓赤霉发酵液中赤霉酸含量
Quick Detection of Gibberellins in Gibberella fujikuroiF ermentation Broth Using Folin-Ciocalteu’s Phenol Reagent
周圣骄 1宋志远 2聂志奎1
作者信息
- 1. 江西新瑞丰生化有限公司，江西新干331300; 江西省植物生长调节剂工程研究中心，江西新干331300
- 2. 江西新瑞丰生化有限公司，江西新干331300
- 折叠
摘要
[目的]优化福林试剂法的反应条件。[方法]以从紫外诱变样品中筛选获得的1株高产藤仓赤霉菌（ G．fujikuroi） F12为研究对象，采用福林试剂法，通过溶液吸光值的变化定量检测藤仓赤霉菌发酵液中赤霉酸（GA3）的产量。[结果]当福林试剂的用量为1．5 mL、盐酸用量为3．0 mL、加热时间为20 min时，所得溶液吸光值稳定性最好，在4 h以内基本保持不变。[结论]福林试剂法操作简便、检测快速准确，可用于藤仓赤霉菌发酵液中GA3的快速检测。
Abstract
Objective] The aim was to optimize reaction conditions of Folin-Ciocalteu’s phenol reagent.[Method] With high-yield Gibberella fujikuroi F12 as the research strain which was isolated from large quantities of UV mutation samples, Folin-Ciocalteu’ s phenol reagent method was used to quickly detect the gibberellin acid ( GA3 ) yield in Gibberella fujikuroi fermentation broth by the change of absorbance.[ Result] When 1.5 mL Folin-Ciocalteu’s phenol reagent, 3.0 mL hydrochloric acid as well as 20 min heating time was selected, the final solution absor-bance had the best stability and still remained basically unchanged within 4 h.[Conclusion] The optimized method can quickly and accurately detect the GA3 yield in Gibberella fujikuroi fermentation broth.
关键词
赤霉酸/福林试剂/快速检测法/藤仓赤霉菌/吸光值Key words
GA3/Folin’s reagent/Quick detection/Gibberella fujikuroi/Absorbance引用本文复制引用
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据