摘要
[目的]建立水产品中诺氟沙星、环丙沙星、恩诺沙星3种氟喹诺酮类药物残留测定的超高效液相色谱法。[方法]以0．10％甲酸－乙腈为提取溶剂，提取液经浓缩，正己烷净化去脂，采用超高效液相色谱－荧光检测器检测3种氟喹诺酮类药物，外标法定量。[结果]该方法的相对标准偏差为1．4％～8．1％，回收率为82％～106％，3种氟喹诺酮类药物在0．01～1．00μg／mL的浓度范围内具有良好的线性关系，相关系数均大于0．995，方法检出限为5．00μg／kg。[结论]该检测方法准确、快捷、高效，可广泛应用于水产品中氟喹诺酮类药物残留的检测。
Abstract
Objective]An ultra performance liquid chromatography method was developed for the simultaneous determination of 3 quinolone antibiotics in norfloxacin, ciproxacin, enrofloxacin.[Method] The method with 0.10% formic acid and acetonitrile as extraction solvent, the extract was concentrated and degreased by hexane, 3 kinds of quinolones antibiotics were detected by ultra-liquid chromatographic with fluores-cence detector, and were quantified using external standard method.[Result] The relative standard deviations(RSD) were 1.4%-8.1%;recoveries were 82%-106%;a variety of quinolone antibiotics showed good linearity in the concentration range of 0.01-1.00 μg/mL, cor-relation coefficients were greater than 0.995;detection limits were 5.00 μg/kg.[Conclusion] The detection method is accurate, fast and effi-cient, and can be widely used in the daily detection of quinolone antibiotics in aquatic products.
国家科技期刊平台
NSTL
维普
万方数据