气相色谱法测定蝉花中角鲨烯的含量
Determination of Squalene in Cordyceps cicadae Shing by Gas Chromatography
凡军民 1贾君 1谢春芹 1韩艳丽1
作者信息
- 1. 江苏农林职业技术学院，江苏句容212400
- 折叠
摘要
[目的]建立毛细管气相色谱（ GC）测定蝉花中角鲨烯含量的方法。[方法]采用气相色谱法测定蝉花中角鲨烯，并对不同部位天然蝉花的角鲨烯含量进行比较，确定蝉花中角鲨烯含量测定的最佳条件。[结果]1 g蝉花经超声波提取后，经1 mL 0．5 mol／L的氢氧化钠－甲醇溶液40℃皂化5 min，再经正已烷萃取净化，采用GC法测定角鲨烯含量。该方法在角鲨烯质量浓度为21．43～214．30μg／mL （r＝0．9992）范围内线性关系良好，方法精密度良好（RSD ＝3．71％），加标回收率为77．80％～97．32％，平均加标回收率（n＝9）为88．10％。[结论]气相色谱法简单快速，污染小，检测灵敏度高，可用于蝉花中角鲨烯含量的测定。
Abstract
Objective] To establish a method of capillary gas chromatography to detect the content of squalene in Cordyceps cicadae.[Method] Squalene in Cordyceps cicadae Shing was detected by gas chromatography.And the squalene contents in different parts of C.cicadae were com-pared.The optimal condition for the detection of squalene in C.cicadae was obtained.[Result] C.cicadae (1 g) was extracted by ultrasonic ex-traction,the product was methyl esterified at 40 ℃for 5 min by 1 mL of 0.5 mol/L sodium hydroxide-methanol solution and then extracted by n-hexane.The squalene could be determined quantitatively by GC.When the squalene concentration was from 21.43 to 214.30 μg/mL,there was a good linear relation (r=0.999 2) and a perfect method precision (RSD =3.71%),and the recovery rate was 77.80%-97.32%,and the aver-age recovery rate was 88.10% (n=9).[Conclusion] This method was simple and rapid with low pollution and high sensitivity,and it is suitable for determination of squalene content in C.cicadae.
关键词
蝉花/角鲨烯/气相色谱法Key words
Cordyceps cicadae Shing/Squalene/Gas chromatography引用本文复制引用
基金项目
江苏农林职业技术学院科研基金(2015 kj034)
创新团队科研基金(2014td002)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据