摘要
[目的]优化紫芝菌丝体多糖的提取工艺，提高紫芝菌丝体多糖的得率。[方法]以紫芝菌丝体为原料，以多糖得率为考察指标，首先对紫芝菌丝体多糖的提取温度、料液比、提取时间、pH、提取次数单因素进行优化，然后采用L9（34）正交试验探索最佳提取工艺。[结果]在料液比1∶30 g／mL，温度90℃，浸提时间4 h，溶液pH 7的条件下，紫芝菌丝体多糖得率最佳，为9．56％。[结论]该研究可为紫芝菌丝体多糖的工业生产提供有利的条件，为紫芝菌丝体多糖的进一步开发利用提供依据。
Abstract
Objective] To optimize the extraction technology for mycelia polysaccharides from Ganoderma sinensis, and to enhance the extrac-tion rate of mycelia polysaccharides.[ Method] With G.sinensis mycelia as the raw materials, we optimized the extraction temperature, solid-liquid ratio, time, pH and extraction times for the extraction of polysaccharides, with the extraction rate of polysaccharides as the investigation index.Then, the optimal extraction technology was researched by L9(34) orthogonal test.[Result] Under the conditions of 1∶30 g/mL solid-liquid ratio,90 ℃extraction temperature , 4 h extraction time pH 7 and adding NaOH, the extraction rate of polysaccharides from G.sinensis mycelia was the optimal, which was 9.56%.[Conclusion] This research provides favorable conditions for the industrial production of mycelia polysaccharides from G.sinensis, and offers experimental basis for the further development and utilization of mycelia polysaccharides from G.sinensis.
基金项目
广东省高等学校科技创新重点项目(2012CXZD0009)
国家自然科学基金(31201330)
广州市科技攻关项目(201300000202)
国家科技期刊平台
NSTL
维普
万方数据