Objective] The aim was to screen out the optimal primary culture, subculture and rooting culture medium for Prinsepia sinensis tissue culture.[ Method] With single bud stem segment of Prinsepia sinensis as explant, using MS or 1/2 MS as the basic culture medium, supplemented with different concentrations of 6-BA, IBA, NAA and GA3 , effects of different culture mediums on bud induction, proliferation and rooting were discussed.[ Result] IBA concentration influenced significantly on starting rate of primary culture of Prinsepia sinensis, the or-der of influence degree was IBA concentration>GA3 concentration>6-BA concentration;6-BA concentration had obvious influence on prolif-eration coefficient of subculture medium, the order of influence degree was 6-BA concentration>IBA concentration>NAA concentration;IBA concentration had a significant effect on rooting index, while NAA concentration had no significant effect.[ Conclusion] The optimal primary culture, subculture and rooting culture mediums for Prinsepia sinensis tissue culture were: MS +0.5 mg/L 6-BA +0.2 mg/L IBA; MS +1.0 mg/L 6-BA+0.1 mg/L IBA;1/2 MS+2.0 mg/L IBA+0.1 mg/L NAA.
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