超高压高效液相色谱法测定茶酒中茶氨酸含量
Determination of Theanine in Tea Liquor by Ultra High Pressure and High Performance Liquid Chromatography
杨威 1吕超志 1刘峰 1李丹 1曹忠1
作者信息
- 1. 长沙理工大学化学与生物工程学院，微纳生物传感与食品安全检测协同创新中心，湖南长沙410114
- 折叠
摘要
[目的]研究茶酒中茶氨酸含量的分析方法。[方法]以邻苯二甲醛（ OPA）为衍生剂，采用超高压高效液相色谱测定茶酒中的茶氨酸。[结果]检测茶氨酸的线性范围为0．1～100．0μg／mL，线性方程可拟合为Y＝53．57X－41．664，相关系数r为0．99959，该方法的最低检出限达到0．05μg／g（以S／N＝3计）。样品中茶氨酸的含量为0．25 mg／kg，加标试验的回收率在99．6％～100．1％。[结论]该方法快速、简单、定量准确，可在茶氨酸的测定分析中推广应用。
Abstract
Objective] The analytical method for content of theanine in tea liquor was investigated.[Method] Using o-phthalaldehyde (OPA) as a derivating agent,a method was established for determination of theanine in tea liquor by super high-pressure high performance liquid chromatog-raphy.[Result] The results showed that the linear range of 0.1-100.0 μg/mL for theanine detection was obtained,and the corresponding linear equation was fitted as Y=53.57X-41.664 with correlation coefficient r of 0.999 59.Also,the minimum detection limit of 0.05 μg/g (S/N=3) was reached.The theanine content of the sample was determined as 0.25 mg/kg with a recovery rate of 99.6%-100.1%.[Conclusion] The method is rapid,simple and accurate,which is easy to be widely used in the determination and analysis of theanine in tea liquor .
关键词
超高压/高效液相色谱/茶酒/茶氨酸Key words
Ultra high pressure/High performance liquid chromatography/Tea liquor/Theanine引用本文复制引用
出版年
2016
国家科技期刊平台
NSTL
万方数据