何鲁牵牛的组培快繁研究
Study on Tissue Culture and Rapid Propagation of Ipomoea holubii
吕永平 1王燕 1牟豪杰 1汪一婷 1李海营1
作者信息
- 1. 浙江省农业科学院病毒学与生物技术研究所，浙江杭州310021
- 折叠
摘要
[目的]建立何鲁牵牛的组培快繁体系，为其规模化生产提供理论依据。[方法]以何鲁牵牛茎段为外植体，研究何鲁牵牛不定芽增殖和生根的最适培养基。[结果]何鲁牵牛在WPM＋0．3 mg／L 6－BA ＋0．01 mg／L NAA＋0．1 g／L活性炭的启动培养基上成功诱导腋芽抽出，将腋芽转接到WPM＋0．3 mg／L 6－BA ＋0．1 g／L活性炭培养基上进行不定芽的增殖，增殖率达4．30；在WPM ＋0．1 mg／L IBA＋0．1 mg／L NAA＋0．1 g／L活性炭培养基上生根率较高，可达90％。[结论]该研究建立了何鲁牵牛的组培快繁体系，有利于何鲁牵牛种质资源保护和工厂化生产。
Abstract
Objective] To establish tissue rapid propagation system of Ipomoea holubii,and to provide theoretical foundation for its large-scale production.[Method] Taking the stem of Ipomoea holubii as explants,we researched the optimal culture medium for adventitious buds prolifera-tion and rooting of I.holubii.[Result] Axillary buds were induced on WPM+0.3 mg/L 6-BA +0.01 mg/L NAA +0.1 g/L active carbon (AC).The shoots of I.holubii were cultured on WPM +0.3 mg/L 6-BA +1 g/L AC for multiplication,and the multiplication ratio was 4.30. WPM +0.1 mg/L IBA+0.1 mg/L NAA+0.1 g/L AC was the optimal medium for rooting,and the rooting rate was 90%.[Conclusion] This re-search establishes the tissue culture and rapid propagation system of I.holubii,which is helpful to the germplasm protection and industrial produc-tion of I.holubii.
关键词
何鲁牵牛/组培快繁/增殖/生根Key words
Ipomoea holubii/Rapid propagation/Multiplication/Rooting引用本文复制引用
基金项目
国际先进农业科技术计划(948)项目(2011－G31)
浙江省农业科学院青年人才培养项目(2015R21R08E08)
出版年
2016
国家科技期刊平台
NSTL
万方数据