蜂蜜中磺胺类和氯霉素类同时测定的前处理方法研究
Study on Pretreatment Method for Simultaneous Determination of Chloramphenicol and Sulfanilamide Grous in Honey
仲光凤 1刘晓玲2
作者信息
- 1. 山东中质华检测试验检验有限公司，山东济宁272000
- 2. 济宁市食品药品检验检测中心，山东济宁272007
- 折叠
摘要
[目的]建立同时测定蜂蜜中磺胺类和氯霉素类的超高效液相色谱串联质谱（ UPLC－MS）的新方法。[方法]试验以0．2％的甲酸乙腈作为提取剂，通过一种新型的小柱（ Oasis ?PRiME HLB柱）净化处理，流动相定容过膜后上机待测，可以同时提取出蜂蜜中的磺胺类和氯霉素类。[结果]氯霉素在0．1～10．0 ng／mL 范围内有良好的线性关系，相关系数大于0．991；磺胺类在0．5～50．0 ng／mL范围内线性关系良好。氯霉素样品最低检出限为0．1μg／kg，磺胺类检出限为0．5μg／kg，回收率在82．50％～101．20％，相对标准偏差为3．2％～4．0％。[结论]该方法快速、准确、灵敏度高，适用于蜂蜜中氯霉素类和磺胺类的检测。
Abstract
Objective] To establish a new method UPLC-MS for the determination of sulfanilamide grous and chloramphenicols in honey . [Method] With 0.2% formic acid acetonitrile as the extraction agent, through purification treatment by a new column ( Oasis? PRiME HLB), sulfanilamide grous and chloramphenicols can be extracted simultaneously.[Result] Chloramphenicol had a good linear relationship in the range of 0.1-10.0 ng/mL, and the correlation coefficient was greater than 0.991; sulfanilamide grous had a good linear relationship in the range of 0.5 -50.0 ng/mL.The lowest detection limit of chloramphenicol was 0.1 g/kg, detection limit of sulfanilamide grous was 0.5 μg/kg, recovery was 82.5%-101.2%, relative standard deviation was 3.2% -4.0%.[Conclusion] The method is rapid, accurate and sensitive, and it is suitable for the detection of chloramphenicol and sulfanilamide grous.
关键词
超高效液相色谱串联质谱/磺胺类/氯霉素类/蜂蜜Key words
UHPLC-MS/Sulfanilamide grous/Chloramphenicols/Honey引用本文复制引用
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据