摘要
[目的]探讨烟草在采收前的生理期和烘烤过程中的变黄阶段抗逆机制。[方法]利用生物信息学方法对烟草bZIP转录因子BZI-4的理化特性、信号肽、跨膜、系统进化和亚细胞定位进行分析。[结果]烟草bZIP转录因子BZI-4为碱性、水溶性、耐高温和非分泌型的游离蛋白,该蛋白为叶绿体中的转运肽,仅剪切修复后才具有活性,对环境适应能力强。[结论]通过烟草bZIP转录因子BZI-4研究,为烟草抗逆境胁迫研究提供理论基础。
Abstract
Objective] To discuss the related factors of resistance to stress in the physiological period of tobacco before harvesting and baking yellowing stage.[Method] The physical and chemical properties, signal peptide, transmembrane, system evolution and subcellular localiza-tion of tobacco bZIP transcription factor BZI-4 were analyzed by bioinformatics methods.[ Result] Tobacco bZIP transcription factor bzi-4 was alkaline water solubility and relative stability, high heat resistance, non-secretion type of free protein.The protein might be existed in chloro-plast transit peptide, its activity must be exerted through the amino acid sequence of shear, and the condition of adaptive evolution was moder-ate.[ Conclusion] The study of bZIP transcription factor BZI-4 in tobacco provides a theoretical basis for the study of tobacco resistance to stress.