摘要
[目的]探讨冰砂糖寿组培快繁的最佳条件。[方法]以冰砂糖寿幼嫩花序为外植体，研究冰砂糖寿组培快繁技术。[结果]于MS ＋5 mg／L 6－BA ＋0．5 mg／L IBA＋30 g／L蔗糖＋7g ／L琼脂的诱导培养基上成功诱导出愈伤组织，在分化培养基MS＋0．3mg ／L 6－BA＋30 g／L蔗糖＋7 g／L琼脂上继代培养可分化成不定芽，将不定芽转接到增殖培养基MS ＋0．3 mg／L 6－BA ＋0．1 mg／LNAA＋30 g／L蔗糖＋7 g／L琼脂上增殖速度较快，增殖系数达3．79。将其移栽至营养基质（混合泥炭∶粗砂＝1∶1）中，移栽成活率可达95％。[结论]建立了冰砂糖寿花序再生及快繁体系，为其规模化生产提供理论依据。
Abstract
Objective]The aim was to study tissue culture and rapid propagation of Hawo rthia ek gazato.[Method]With young inflorescence of Haworthia kegza ato as explant,tissue culture and rapid propagation of Haworthai kegazato was studied.[Result] Embryogenic callus was initia-ted from Hawo tr hia comptoniana ×H. oc rrecta cv.'Aboukyu i nflorse cence cultured on MS medium supplemented with 5 mg/L 6-BA, 0.5mg /L IBA, 30g /L sucrose, and 7g/L agar.Subculture of these embryogenic calli on the MS +0.3 mg/L 6-BA+30 g/L sucrose+7 g/L agar medi-um resulted in shoots and micropropagation of shoots on the MS +0.3 mg/L 6-BA+0.1 mg/L NAA +30 g/L sucrose7 g/L agar medium with muitiplication coefficient of 3.79.The transplanting survival rate was 95%in nutritional substrates.[Conclusion] A subsequent plant regeneration from inflorescence callus of Hawo rthiak egazaot were established,to provide theoretical basis for large -scale production.
国家科技期刊平台
NSTL
维普
万方数据