摘要
[目的]对湖北咸宁地区山坡土样中分离得到的1株放线菌HBERC－31270的发酵提取物进行快速鉴定及活性评价。[方法]对HBERC－31270进行发酵与提取，并对发酵提取物进行生物活性测定、高效液相色谱－质谱（HPLC－MS）、紫外吸收光谱（UV）分析及活性评价。[结果]提取物高效液相色谱（HPLC）制备组分活性测定结果表明，其活性部位在14～21 min。根据HPLC－MS及UV确定其主要成分为一个小分子化合物。通过对其质谱数据进行分析确定了分子离子峰，经天然产物数据库比对及文献调研，确定其主要活性成分为9－甲基链米酮。盆栽试验结果表明，该菌对多种作物真菌性病害具有较好的防治效果。[结论]试验结果为放线菌HBERC －31270的进一步研究提供了理论依据。
Abstract
Objective] Rapid identification and activity evaluation were carried out for the fermentation extracts of a strain HBERC-31270 which was isolated from a soil sample collected in Xianning,Hubei province.[Method] The strain HBERC-31270 was fermented and extracted,and bio-activity determination,HPLC-MS,UV spectrum analysis and activity evaluation were carried out for the fermentation extracts.[Result] It was de-termined by components bioassay that the active compounds were in between 14-21 min in HPLC profile,and the compounds were identified as 9-methylstreptimidone on the basis of MS,UV absorption spectrum and natural product dictionary.It was found by pot test that the fermentation broth was very effective against a variety of fungal diseases.[Conclusion] The results provide theoretical basis for the further study of actinomyce-tes HBERC-31270 .
基金项目
湖北省农业科技创新中心项目(2016－620－000－001－039)
国家科技期刊平台
NSTL
维普
万方数据