生石花植株离体再生及组培快繁研究
Plantlet Regeneration in vitro and Rapid Propagation of Lithops sp.
牟豪杰 1王燕 1吕永平 1汪一婷 1李海营1
作者信息
- 1. 浙江省农业科学院病毒学与生物技术研究所，浙江杭州310021
- 折叠
摘要
[目的]建立生石花植株离体再生及组培快繁体系。[方法]以生石花成熟种子为外植体，研究生石花组培快繁技术。[结果]萌发后的幼苗在MS＋0．50 mg／L 6－BA＋0．05 mg／L NAA培养基上被成功诱导出愈伤组织；不添加6－BA的MS培养基有利于愈伤组织的分化，不定芽诱导率为4．65；在MS ＋0．05 mg／L 6－BA＋0．01 mg／L NAA培养基上不定芽增殖率较高，可达5．60。[结论]建立了生石花植株离体再生及快繁体系，有利于生石花种质资源保护和工厂化生产。
Abstract
Objective] The aim was to establish plantlet regeneration in vitro and rapid propagation of Lithops sp.[Method] With mature seeds as explant, rapid propagation technique of Lithops sp.was studied.[ Result] Calli were induced from the young seedling on MS medium +0.50 mg/L 6-BA+0.05 mg/L NAA in this study.The results showed that the basic MS medium without 6-BA benefited the callus differentiation of Lithops sp., and the induction ratio of adventitious bud was 4.65;MS+0.05 mg/L 6-BA+0.01 mg/L NAA was the optimal medium for the multiplication of adventitious bud, and the multiplication ratio was 5.60.[Conclusion] The plantlet regeneration and rapid propagation system of Lithops sp.were established, which has important guiding significance for the germplasm protection and industrial production of Lithops sp..
关键词
生石花/愈伤组织/离体再生/增殖/生根Key words
Lithops sp./Callus/Regeneration in vitro/Proliferation/Rooting引用本文复制引用
基金项目
引进国际先进农业科学技术计划（948）项目(2011－G31)
浙江省农业科学院青年人才培养项目(2015R21R08E08)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据