帚石楠组培快繁研究
Tissue Culture and Rapid Propagation of Calluna vulgaris
孙丽娜 1吴春华 1王淼1
作者信息
- 1. 大连农业科学研究院,辽宁大连116036
- 折叠
摘要
[目的]建立帚石楠组织培养快速繁殖技术.[方法]以帚石楠为研究对象,从不同消毒方式及时间、不同生长调节剂配比、不同暗处理时间等方面研究帚石楠的组培快繁体系.[结果]处理帚石楠幼嫩茎段时,以0.1％升汞处理12 min较为适宜;最适的分化培养基为MS+ BA 0.5 mg/L+ 1.0 mg/L2,4-D;最适生根培养基为MS+ IBA 0.15 mg/L+ NAA0.25 mg/L;接种后将接种苗进行5d暗处理可明显提高生根率.[结论]建立了帚石楠组织培养快速繁殖技术体系,为其规模化生产提供理论依据.
Abstract
[Objective] The aim was to establish tissue culture and rapid propagation of Calluna vulgaris.[Method] This experiment was conducted to investigate in vitro micropropagation of Calluna vulgaris with the aptical buds excised from the mature elite plants.[Result]The results showed that the optimum disinfecting time was 12 min with 0.1％ Mercuric chloride solution;and the optimum medium of following proliferation was MS + BA 0.5 mg/L + 2,4-D 1.0 mg/L;When the MS + IBA0.15 mg/L + NAA 0.25 mg/L medium induced rooting;Culturing in dark for 5 days coluld promoted rooting.[Conclusion] The study establish tissue culture and rapid propagation of Calluna vulgaris,and provide theoretical basis for the mass production.
关键词
帚石楠/组织培养/暗培养Key words
Calluna vulgaris/Tissue culture/Rapid propagation引用本文复制引用
基金项目
大连市科学技术局科技计划项目(2014B11NC076)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据