一步获取基因定点修饰技术测序样品双峰中突变序列方法的建立
One Step Analysis of Mutation Sequences in Two Peak Sequencing Samples Obtained by the Site-directed Mutagenesis
赖娅娜 1周洲2
作者信息
- 1. 南京医科大学,江苏南京211166
- 2. 南京师范大学,江苏南京210023
- 折叠
摘要
介绍了一种经济高效获取突变序列的方法.该方法克服了传统的基因定点突变样品双峰测序峰图分析工作量大、操作繁琐、结果判定周期长等问题,通过与野生型序列的对比分析,判定突变类型,直接获取突变型基因序列,从而为基因定点突变技术中快速筛选、确定阳性样品及其研究奠定理论基础.
Abstract
A quick method to obtain the sequence of mutation was mtroduced.The traditional sequence analysis method of site-directed gene mutagenesis had the problems such as complicated operation,long result determination cycle and high cost of research.Mutations in the sequence and the type of mutation could be obtained by direct comparison with the wild type sequence.As a result,this method could obviously improve the work efficiency and also provided an economical and etficient sequence analysis for the selection of positive samples of gene site mutation technique for researchers.
关键词
序列分析/基因定点修饰技术/突变序列Key words
Sequence analysis/The site-directed mutagenesis/Mutatim sequence引用本文复制引用
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据