取样时间和消毒方法对青钱柳茎段腋芽诱导的影响
Effects of Sampling Time and Disinfection Methods on Axillary Bud Induction of Cyclocarya paliurus
王纪 1杨静 2杨万霞 2方升佐2
作者信息
- 1. 南京晓庄学院,江苏南京211171
- 2. 南京林业大学,江苏南京210037
- 折叠
摘要
[目的]解决青钱柳组织培养过程中外植体污染问题.[方法]以青钱柳带腋芽的茎段为材料,研究不同取样时间和消毒方法对青钱柳茎段腋芽萌发率的影响.[结果]最佳采样时间为4、5月,最优消毒处理分别为70％乙醇30 s+0.10％升汞12 min(4月)和70％乙醇30s+0.05％升汞16 min(5月).[结论]试验结果为建立青钱柳的无性快繁体系奠定了基础.
Abstract
[Objective] The aim was to solve the problem of explants pollution in tissue culture of Cyclocarya paliurus.[Method] Axillary bud from stem segments of Cyclocarya paliurus were used as explants,and the effects sampling time and disinfection methods on axillary bud induction of Cyclocarya paliurus were studied.[Result] The best sampling time was April and May,70％ alcohol 30 s + 0.10％ mercuric chloride 12 min for April and 70％ alcohol 30 s +0.05％ mercuric chloride 16 min for May.[Conclusion] The results lay the basis for establishing asexual micropropagation system of Cyclocarya paliurus.
关键词
青钱柳/取样时间/消毒/腋芽诱导Key words
Cyclocarya paliurus/Sampling time/Disinfection methods/Axillary bud induction引用本文复制引用
基金项目
国家自然科学基金(31270673)
南京晓庄学院青年专项(2015NXY76)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据