泰妙菌素生产菌种原生质体制备条件优化
Optimization on Protoplast Preparation Conditions of Tiamulin Producing Strain
杨智 1孙金刚 1牛春 1张萍1
作者信息
- 1. 宁夏泰瑞制药股份有限公司,宁夏银川750101
- 折叠
摘要
[目的]探讨泰妙菌素生产菌种原生质体的最优制备方法.[方法]通过对原生质体制备条件进行优化,建立泰妙生产菌种原生质体制备体系.[结果]优化后制备条件:以培养3d的菌丝体为材料,将其置于含质量浓度0.10％溶壁酶、0.60 mol/L MgSO4的酶液中,25℃酶解3.0～3.5 h,将酶解液用G2砂芯漏斗过滤,滤波即为原生质体悬液.采用该方法,泰妙生产菌种原生质体制备率达1.5×106个/mL.[结论]试验结果为提高泰妙菌素生产菌种的产量提供了理论依据.
Abstract
[Objective] The aim was to explore the optimal protoplast preparation method of Tiamulin producing strain.[Method] We established a system for protoplast preparation of Tiamulin producing strain by optimizing protoplast preparation conditions.[Result] The opotimal conditions were as followed:used 3 days cultured mycelium as material;put it into a solution which contain 0.10％ lyticase,0.60 mol/L MgSO4;hydrolyzed the mycelium with enzyme under 25 ℃ for 3.0-3.5 hours,then fihered the solution with G2 sintered discs;the filter liquor was protoplast suspemion.By using this method,the protoplast preparation rate of Tiamulin producing strain could reach 1.5 × 106 per milliliter.[Conclusion] The results provide theoretical basis for the improvement of Tiamulin producing strain.
关键词
泰妙菌素/原生质体/溶壁酶Key words
Tiamulin/Protoplast/Lyticase引用本文复制引用
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据