不同培养基对黑木耳锁状联合菌丝比率的影响
Effects of Culture Medium on Clamp-connection Hyphae Ratio of Aurieuzaria auricular
王妍 1杜春梅 2姜忠元 3董锡文2
作者信息
- 1. 佳木斯大学生命科学学院,黑龙江佳木斯154007
- 2. 佳木斯大学生命科学学院,黑龙江佳木斯154007;佳木斯大学微生物研究所,黑龙江佳木斯154007
- 3. 佳木斯大学教务处,黑龙江佳木斯154007
- 折叠
摘要
[目的]研究培养基中葡萄糖、蛋白胨、丙氨酸对黑木耳锁状联合菌丝比率的影响.[方法]采用平板插片培养方法,以黑木耳H29为试验材料,通过单因素试验确定葡萄糖、蛋白胨、丙氨酸对锁状联合菌丝比率促进的最佳浓度.再通过正交试验确定其最佳组合.[结果]适当浓度的葡萄糖、蛋白胨、丙氨酸均能提高H29锁状联合菌丝比率,其最佳组合为20 mg/mL葡萄糖、6mg/mL蛋白胨、3.5 mg/mL丙氨酸.[结论]该研究为黑木耳的育种和实际生产提供参考.
Abstract
[Objective] To study the effects of glucose,peptone and alanine in culture medium on clamp-connection hyphae ratio of Aurieuzaria auricular.[Method] With A.auricular H29 as the test materials,plate culture method was adopted.The optimal concentrations of glucose,peptone and alanine that promoting the clamp-connection hyphae ratio were determined by single factor test.The optimal constitution was determined by orthogonal test.[Result] The appropriate concentrations of glucose,peptone and alanine could improve the clamp-connection hyphae ratio of A.auricular H29.The optimal concentration combination was 20 mg/mL glucose,6 mg/mL peptone and 3.5 mg/mL alanine.[Conclusion] This research provides references for the breeding and practical production of A.auricular.
关键词
黑木耳/锁状联合/培养基Key words
Aurieuzaria auricular/Clamp connection/Culture medium引用本文复制引用
基金项目
佳木斯大学校长创新创业基金研发项目(xzyf2014-17)
佳木斯大学研究生科技创新项目(LZZ2015-007)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据