熟地黄多糖含量测定方法研究
Study on the Determination Methods of Polysaccharide Content of Radix Rehmanniae Preparata
吴诗云 1钟益宁 1张焱 1柳欢 1杨丽声 1梁礼群1
作者信息
摘要
[目的]建立熟地黄中多糖含量的测定方法.[方法]利用苯酚硫酸法、蒽酮硫酸法、3,5-二硝基水杨酸法(DNS法)分别测定熟地黄中的多糖含量,对检测结果进行比较.[结果]苯酚硫酸法、蒽酮硫酸法、DNS法的平均回收率分别为100.5％、99.8％、100.6％,RSD分别为1.6％、1.6％、1.5％,测得的多糖质量分数分别为14.80、14.66、17.46 mg/g.[结论]苯酚硫酸法的结果更可靠,且操作相对简单易行,可考虑作为熟地黄多糖含量测定的首选方法.
Abstract
[Objective] The research aimed to estabhsh the determination method of polysaccharide content of Radix Rehmanniae Preparata..[Method] The polysaccharide contems of Radix Rehmanniae Preparata.were determined by phenol-sulfuric acid method,anthrone-sulfuric acid method and 3,5-dinitrosalicylic acid method (DNS method) respectively,and the test results were compared.[Result] The average recovery rates of phenol-sulfuric acid method,anthrone-sulfuric acid method and DNS method were 100.5％,99.8％ and 100.6％,respectively.The RSD were 1.6％,1.6％ and 1.5％ respectively.The mass fraction of polysaccharides were 14.80,14.66 and 17.46 mg/g,respectively.[Conclusion] The phenol-sulfuric acid method was more reliable,the operation was relatively simple,which can be considered as the preferred method of determination of oolvsaccharide content of Radix Rehmanniae Preparata.
关键词
熟地黄/多糖/含量测定/苯酚硫酸法/蒽酮硫酸法/3,5-二硝基水杨酸法Key words
Radix Rehmanniae Preparata./Polysaccharide/Content determination/Phenol-sulfuric acid method/Anthrone-sulfuric acid method/3,5-dinitrosalicylic acid method引用本文复制引用
基金项目
广西科技攻关计划项目(桂科攻1346008-4)
广西自然科学基金(2014GXNSFAA118271)
2014年博士研究生教育发展项目(050140002)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据