摘要
探讨了以马铃薯茎段、叶片及块茎为起始外植体不同离体再生体系的建立和相关影响因素,分析了基因型、受体外植体类型、菌液浓度、侵染时间、共培养和预培养等对遗传转化效率的影响,为建立马铃薯高效离体再生体系和遗传转化体系提供理论参考。
Abstract
The establishment of different potato in vitro regeneration systems and related impact factors were discussed with stem ,leaf and tuber as initial explant;and the effects of genotype,type of receptor system,bacterial concentration,infection time,co-culture and pre-culture on genetic transformation efficiency were analyzed, so as to provide important reference for establishment of high efficiency in vitro regeneration and genetic transformation system in potato.
基金项目
复旦大学遗传工程国家重点实验室开放课题(SKLGE -1410)
国家高技术研究发展计划“863”计划(2013AA102704)