响应面法优化泰妙菌素生产菌株原生质体再生培养基
Optimization of Protoplast Regeneration Medium for the Producing Bacteria of Tiamulin by Using Response Surface Methodology
孙金刚 1杨智 1牛春 1张萍1
作者信息
- 1. 宁夏泰瑞制药股份有限公司，宁夏银川750101
- 折叠
摘要
[目的]采用响应面法对泰妙菌素生产菌株原生质体的再生培养基进行优化。[方法]利用Minitab统计软件，采用中心复合响应面设计对泰妙菌素生产菌株原生质体再生培养基的琼脂浓度和蔗糖浓度进行了优化。[结果]泰妙菌素生产菌株原生质体的再生培养基优化为马铃薯20．00％（浸提液）＋葡萄糖2．00％＋蔗糖2．70％＋琼脂1．56％，pH＝6．5。优化后的再生培养基的单菌落复苏率由0．7‰提高到3．5‰。[结论]该研究可为泰妙菌素的研发奠定基础。
Abstract
Objective] To optimize the regeneration medium of protoplast for the producing bacteria of tiamulin by using response surface meth-odology.[ Method] The concentrations of agar and sucrose in the regeneration medium of protoplast for the producing bacteria of tiamulin were optimized by using central composite response surface design and Minitab software .[ Result] The regeneration medium of protoplast for the pro-ducing bacteria of tiamulin was optimized as 20.00%potato extract +2.00%glucose +2.70%sucrose +1.56%agar, pH=6.5.The regen-eration rate of recovered colony increased from 0.7‰ to 3.5‰.[Conclusion] The research can lay the foundation for the development and re-search of tiamulin.
关键词
响应面法/泰妙菌素/原生质体/再生培养基Key words
Response surface methodology/Tiamulin/Protoplast/Regeneration medium引用本文复制引用
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据