超声波法提取凹叶厚朴内生真菌代谢产物
Ultrasonic Extraction of Magnolia officinalis Endophytic Fungi Metabolites
耿直1
作者信息
- 1. 陕西国际商贸学院,陕西西安712046
- 折叠
摘要
[目的]优化超声波法提取凹叶厚朴内生真菌代谢产物的工艺.[方法]采用正交试验设计,以超声温度、超声时间、超声功率为考察因素,以厚朴酚的含量为指标,采用高效液相色谱法,确定发酵液的最佳超声提取条件.[结果]最佳超声提取条件为超声温度50℃、超声时间60 min、超声功率100 kHz.[结论]该方法能够用于内生真菌发酵液中代谢产物提取.
Abstract
[Objective] The aim was to optimize the technique for extracting endogenous fungal metabolites from Magnolia officinalis with ultrasonic method.[Method] Using orthogonal test design,with ultrasonic temperature,ultrasonic time,ultrasonic power as investigated factors,the content of magnolol as indicator,adopting HPLC method,the optimum ultrasonic extraction conditions of mycelia were obtained.[Result] The optimum ultrasonic extraction conditions were as following:ultrasonic temperature 50 ℃C,ultrasonic time 60 min,ultrasonic power 100 kHz.[Conclusion] The method can be used to extract endogenous fungal metabolites in the fermented liquid.
关键词
凹叶厚朴/内生真菌/超声提取/正交试验Key words
Magnolia officinalis/Endophytic fungi/Ultrasonic extraction/Orthogonal test引用本文复制引用
基金项目
陕西省教育厅2014年科学研究计划(专项项目(14JK2010)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据