首页|铁皮石斛根系促生内生真菌的筛选

铁皮石斛根系促生内生真菌的筛选

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[目的]开展铁皮石斛根系促生内生真菌的筛选,为铁皮石斛的批量化生产提供生物防治基础.[方法]采用组织分离法对铁皮石斛根系内生真菌进行分离纯化,再通过单一回接试验将已分离内生真菌回接到铁皮石斛组培苗中,40d后观察铁皮石斛根系生长情况,60d后统计成活率.通过铁皮石斛根系生长指标,筛选出对根系促生作用最显著的内生真菌.[结果]从铁皮石斛根系中共分离得到21株内生真菌,筛选出8株优势促生根系内生真菌,其中回接菌株F5的铁皮石斛组培苗生根率最高,达99.00%,回接菌株F14的铁皮石斛苗成活率达100.00%,为对照组的395%.[结论]该研究成功解决了铁皮石斛移栽成活率低的难题.
Selection of Growth Promoting Endophytic Fungi for Dendrobium candidum
[Objective] The aim was to screen out growth promoting endophytic fungi for Dendrobium candidum,to provide the basis for biological control of batch production of Dendrobium candidum.[Method] Endophytic fungi were isolated and purified from Dendrobium candidum roots by tissue isolation method.Through single inoculation experiment,the isolated endophytic fungi were inoculated to tissue culture seedlings.After 40 d,roots growth was observed,the survival rate was calculated after 60 d.By observing root growth index,endophytic fungi with most significant growth promoting effect were screened out.[Result] 21 strains of endophytic fungi were isolated from Dendrobium candidum roots,among which,8 dominant endophytic fungi were screened out.The rooting rate of tissue culture seedling of strain F5 was the highest,reaching 99.00%,the survival rate of F14 was up to 100.00%,which is 395% of the control group.[Conclusion] The low survival rate of transplanting of Dendrobium candidum was successfully solved.

Dendrobium candidumEndophytic fungiScreeningRoot growthSurvival rate

李昆华、杨丹、苏娟、李锋、岳健

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云南山里红生物科技有限公司,云南昆明650206

铁皮石斛 内生真菌 筛选 根系生长 成活率

昆明市官渡区科技计划项目

官科计字2015-04

2017

安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子:0.413
ISSN:0517-6611
年,卷(期):2017.45(1)
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