铁皮石斛根系促生内生真菌的筛选
Selection of Growth Promoting Endophytic Fungi for Dendrobium candidum
李昆华 1杨丹 1苏娟 1李锋 1岳健1
作者信息
- 1. 云南山里红生物科技有限公司,云南昆明650206
- 折叠
摘要
[目的]开展铁皮石斛根系促生内生真菌的筛选,为铁皮石斛的批量化生产提供生物防治基础.[方法]采用组织分离法对铁皮石斛根系内生真菌进行分离纯化,再通过单一回接试验将已分离内生真菌回接到铁皮石斛组培苗中,40d后观察铁皮石斛根系生长情况,60d后统计成活率.通过铁皮石斛根系生长指标,筛选出对根系促生作用最显著的内生真菌.[结果]从铁皮石斛根系中共分离得到21株内生真菌,筛选出8株优势促生根系内生真菌,其中回接菌株F5的铁皮石斛组培苗生根率最高,达99.00％,回接菌株F14的铁皮石斛苗成活率达100.00％,为对照组的395％.[结论]该研究成功解决了铁皮石斛移栽成活率低的难题.
Abstract
[Objective] The aim was to screen out growth promoting endophytic fungi for Dendrobium candidum,to provide the basis for biological control of batch production of Dendrobium candidum.[Method] Endophytic fungi were isolated and purified from Dendrobium candidum roots by tissue isolation method.Through single inoculation experiment,the isolated endophytic fungi were inoculated to tissue culture seedlings.After 40 d,roots growth was observed,the survival rate was calculated after 60 d.By observing root growth index,endophytic fungi with most significant growth promoting effect were screened out.[Result] 21 strains of endophytic fungi were isolated from Dendrobium candidum roots,among which,8 dominant endophytic fungi were screened out.The rooting rate of tissue culture seedling of strain F5 was the highest,reaching 99.00％,the survival rate of F14 was up to 100.00％,which is 395％ of the control group.[Conclusion] The low survival rate of transplanting of Dendrobium candidum was successfully solved.
关键词
铁皮石斛/内生真菌/筛选/根系生长/成活率Key words
Dendrobium candidum/Endophytic fungi/Screening/Root growth/Survival rate引用本文复制引用
基金项目
昆明市官渡区科技计划项目(官科计字2015-04)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据