Selection of Growth Promoting Endophytic Fungi for Dendrobium candidum
[Objective] The aim was to screen out growth promoting endophytic fungi for Dendrobium candidum,to provide the basis for biological control of batch production of Dendrobium candidum.[Method] Endophytic fungi were isolated and purified from Dendrobium candidum roots by tissue isolation method.Through single inoculation experiment,the isolated endophytic fungi were inoculated to tissue culture seedlings.After 40 d,roots growth was observed,the survival rate was calculated after 60 d.By observing root growth index,endophytic fungi with most significant growth promoting effect were screened out.[Result] 21 strains of endophytic fungi were isolated from Dendrobium candidum roots,among which,8 dominant endophytic fungi were screened out.The rooting rate of tissue culture seedling of strain F5 was the highest,reaching 99.00%,the survival rate of F14 was up to 100.00%,which is 395% of the control group.[Conclusion] The low survival rate of transplanting of Dendrobium candidum was successfully solved.