苦瓜子衣色素蛋白提取及抗氧化能力研究
Study on Antioxidant Effects and Extracting of Momordica charantia Sub-skin Pigment Protein
于群 1张惠 1安磊 1杨浩宇1
作者信息
- 1. 宁夏职业技术学院,宁夏银川750021
- 折叠
摘要
[目的]研究苦瓜子衣色素蛋白的最佳提取工艺及其抗氧化能力,为天然色素的开发应用提供参考.[方法]采用乙酸乙酯法提取苦瓜子衣色素,双水相法提取苦瓜子衣色素蛋白,经DEAE-52阴离子层析、Sephadex G-100凝胶柱层析得到纯化蛋白,参考芬顿法测定其抗氧化能力.[结果]确定苦瓜子衣色素提取的最佳条件为温度50℃,料液比l∶40(g∶ mL),时间30 min,该条件下提取率最高,为45.54％.双水相法中硫酸铵质量分数为35％,聚乙二醇质量分数为30％,提取得到苦瓜子衣色素粗蛋白,经过层析得到分子量为74.10 kD的纯化蛋白.抗氧化试验表明,纯化苦瓜子衣色素蛋白具有抗氧化能力,且随着质量浓度的增加抗氧化性增强.[结论]该试验采用新兴的双水相法提取苦瓜子衣色素蛋白,简化了提取工艺,减少了蛋白质的流失;抗氧化试验为从天然色素中制备高品质无污染的抗氧化制剂奠定了理论基础.
Abstract
[Objective] To study the best condition for the extraction and antioxidant activity of sub-skin pigment from Mordica charantia,to provide a reference for the development and application of natural pigment.[Method] Extracted Momordica charantia sub-skin pigment with ethyl acetate,extracted the protein with aqueous two-phase system,purified it by DEAE-52 anion chromatography and Sephadex G-100 gel column chromatography,determined the antioxidant effects by Fenton method.[Result] The best condition revealed that:temperature 50 ℃,proportion of solid to liquid is 1∶40 (g∶ mL),time 30 min,the extraction rate up to 45.54％.The condition of aqueous two-phase system was:35％ ammonium sulfate and polyethylene glycol of 30％,purified the protein with molecular weight 74.10 kD.The antioxidant tests indicated that Momordica charantia sub-skin pigment protein had antioxidant effects and their eliminate capacity increased with the concentration of solution increased.[Conclusion] This test used a new aqueous two-phase system to extract the protein,simplified the extraction process and reduced the loss of protein.The antioxidant test provided a theoretical basis for the preparation of high quality and non polluting antioxidant preparation from natural pigments.
关键词
苦瓜子衣色素蛋白/分离纯化/柱层析/抗氧化作用Key words
Momordica charantia sub-skin pigment protein/Separation and purification/Column chromatography/Antioxidant引用本文复制引用
基金项目
宁夏职业技术学院(宁夏广播电视大学)科研发展基金()
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据