银杏花粉及其发酵饮料对小鼠的急性毒性评价
Acute Toxic Effects of Ginkgo Pollen and Its Fermentation Beverage on Mice
王未名 1裘纪莹 1刘孝永 1辛雪 1孙华 1陈蕾蕾 1张翔 1王易芬 1陈相艳1
作者信息
- 1. 山东省农业科学院农产品研究所,山东省农产品精深加工技术重点实验室,山东济南250100
- 折叠
摘要
[目的]评价银杏花粉及其发酵饮料的食用安全性.[方法]选择健康小鼠进行急性毒性试验,开展了大剂量预试验及小鼠最大给药量试验,并测定了该剂量下的小鼠胸腺系数和脾脏系数.[结果]大剂量预试验小鼠均未死亡,未有异常反应,不能测出LD50.最大给药量试验显示,小鼠灌胃给药后除银杏花粉组1只小鼠意外死亡外,其他小鼠均未发生死亡或中毒症状,活动、脏器等均未见异常.银杏花粉和银杏花粉发酵饮料的最大给药量分别为21.1 g/kg体重和80.0 mL/kg体重,此最大给药量远高于可能摄入的最高剂量;同时在此剂量下银杏花粉及其发酵饮料能够增加雄性小鼠的胸腺系数和脾脏系数.[结论]银杏花粉及其发酵饮料对小鼠没有急性毒性作用,并且提示对雄性小鼠可能具有一定的免疫增强作用.
Abstract
[Objective] To evaluate the edible safety of ginkgo pollen and its fermentation beverage.[Method] Healthy mice were chosen for acute toxicity test.High-dose pilot study and maximum dose experiment were carried out,as well as determination of the thymus and spleen coefficients of the mice in this dose.[Result] The mice in the high-dose pilot study were all alive,no abnormal reaction,and the LD50 could not be measured.The maximum dose experiment showed that except the accidental death of one mouse,no other death or poisoning symptoms occur,and the activities and organs were all normal.The maximum dose of ginkgo pollen and its fermentation beverage were 21.1 g/kg and 80.0 mL/kg,respectively.These dose were much higher than those could be ingested,and the thymus and spleen coefficients of the mice in this dose were increased.[Conclusion] There are no acute toxic effects of ginkgo pollen and its fermentation beverage on mice,and prompting that they might have some immune enhancement effects on male mice.
关键词
银杏花粉/小鼠/急性毒性/最大给药量Key words
Ginkgo pollen/Mice/Acute toxicity/Maximum administration dosage引用本文复制引用
基金项目
山东省自然科学基金三院联合基金(ZR2014YL020)
山东省自然科学基金三院联合基金(ZR2016YL022)
国家科技支撑计划(2015BAD16B02)
山东省农业科学院青年科研基金(2014QNM47)
泰山学者专项工程项目()
山东省农业科学院青年英才培养计划()
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据