响应面法优化多花勾儿茶果实多酚提取工艺及其抗氧化活性研究
Response Surface Optimized Extraction of Polyphenols from Berchemia floribunda Fruit and the Antioxidants Activities
姚姝凤 1成江 1刘小攀 1唐克华 1董爱文1
作者信息
- 1. 吉首大学林产化工工程湖南省重点实验室,湖南张家界427000
- 折叠
摘要
[目的]优化多花勾儿茶果实中多酚提取工艺,并以叶多酚为对照,考察其果实多酚体外抗氧化能力.[方法]运用Box-BehnkenDesign响应面法优化多花勾儿荼果实提取工艺,并通过测定其总抗氧化能力,清除DPPH·自由基、清除羟基自由基及超氧阴离子自由基清除率考察其抗氧化能力.[结果]最优条件:乙醇体积分数51.66％、料液比1∶35、提取时间2.5h,该条件下多花勾儿茶果实多酚的得率为5.684 0 mg/g.同条件下提取多花勾儿茶果实及叶的多酚,且均具有较强抗氧化能力.[结论]多花勾儿茶果实多酚具有一定清除自由基能力,开发前景广阔.
Abstract
[Objective] The extraction conditions of total polyphenols from the fruits of Berchemiafloribunda were optimized and the antioxidants activities were investigated with the leaves as comparison.[Method] The optimal conditions to achieve the maximum yield of polyphenols were determined by the Box-Behnken design response surface methodology and the antioxidant activities of the total polyphenols were evaluated in vitro by scavenging capabilities of DPPH radical,hydroxyl radical and superoxide radical and total antioxidant capacity.[Result] Under the optimized conditions:ethanol volume fraction 51.66％,solid-liquid ratio 1∶35 and extraction time 2.5 h,the experimental total polyphenols from fruits were 5.684 0 mg/g.And the polyphenols from fruits and leaves under the same extraction conditions both have high antioxidant activities.[Conclusion] Total polyphenols from the fruits of B.floribunda have a certain free radical scavenging capacity with broad prospects for development.
关键词
多花勾儿茶/多酚/响应面优化法/抗氧化Key words
Berchemia floribunda/Polyphenol/Response surface methodology/Antioxidant引用本文复制引用
基金项目
张家界市科技局科技计划项目(2015FJ1149)
吉首大学校级科研项目(15JDY018)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据