软枣猕猴桃原生质体酶解液配方筛选
Screening of Enzymatic Hydrolysate Formula of Actinidia arguta Protoplast
付鹏跃 1刘德江 1申健 1吕明医 1梁雪莹 1董实伟 1程海涛1
作者信息
- 1. 佳木斯大学生命科学学院,黑龙江佳木斯154007
- 折叠
摘要
[目的]筛选软枣猕猴桃原生质体最佳酶解液配方.[方法]以4种配方酶解液在相同条件下处理等量软枣猕猴桃叶片,比较最后收集的纯化原生质体量,筛选最佳酶解液配方.[结果]配方Ⅲ(1.0％纤维素酶、0.5％离析酶、0.3％果胶酶)和Ⅳ(1.0％纤维素酶、0.5％果胶酶)处理酶解效果要明显优于配方Ⅰ(2.0％纤维素酶、0.5％离析酶)、Ⅱ(2.0％纤维素酶、0.3％果胶酶).[结论]配方Ⅲ(1.0％纤维素酶、0.5％离析酶、0.3％果胶酶)为软枣猕猴桃原生质体最佳酶解液配方.
Abstract
[Objective] To screen the best enzymatic hydrolysate formula of Actinidia arguta protoplast.[Method] The leaves of A.arguta were treated with four enzymatic hydrolysate formula under the same conditions.The purified protoplast was collected and compared to screen the optimal enzymatic hydrolysate formula.[Result] The amount of protoplast collected by the solution of formula Ⅲ (1.0％ cellulase,0.5％ segregation enzyme and 0.3％ pectinase) and formula Ⅳ (1.0％ cellulase,0.5％ pectinase)were significantly higher than the solution of formula Ⅰ (2.0％ cellulase,0.5％ segregation enzyme) and formula Ⅱ (2.0％ cellulase,0.3％ pectinase).[Conclusion] Formula Ⅲ (1.0％ cellulase,0.5％ segregation enzyme and 0.3％ pectinase) is the best enzymatic hydrolysate formula for A.arguta protoplast.
关键词
软枣猕猴桃/原生质体/酶解/配方筛选Key words
Actinidia arguta/Protoplast/Enzymatic hydrolysis/Formula screening引用本文复制引用
基金项目
黑龙江省大学生创新项目(201610222104)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据