水稻白叶枯病菌fkbM基因对其运动性的影响
Effect of the fkbM Gene of Xanthomonas oryzae pv.oryzae on Motility
伍甜1
作者信息
- 1. 佛山市高明区明城镇农业技术推广中心,广东佛山528518
- 折叠
摘要
[目的]研究水稻白叶枯病菌fkbM基因对其运动性的影响.[方法]采用同源置换方法对水稻白叶枯病菌野生型菌株PXO99A的fkbM基因进行基因敲除,构建突变菌株△fkbMXoo,同时构建互补菌株△fkbMXoo(C).通过运动性试验,确定fkbM基因对运动性的影响.[结果]与野生型菌株PX099A相比,△fkbMXoo突变体运动性明显减弱,而互补菌株△fkbMXoo(C)能基本恢复水稻白叶枯病菌的运动性.[结论]水稻白叶枯病菌fkbM基因突变能减弱水稻白叶枯病菌的运动能力.
Abstract
[Objective] To study the effect of thefkbM gene of Xanthomonas oryzae pv.oryzae on motility.[Method] A deletion mutant offkbM was generated by homologous recombination △fkbMxoo.To construct a complementary strain △fkbMXoo (C).Using motiliy test experiment to confirm the effect of thefkbM gene on motility.[Result] Compared with wild type strain PXO99A,the mutant △fkbMXoo displayed reduced motility.In complementary strain △fkbMXoo (C),changed phenotypes were restored to certain degree.[Conclusion] ThefkbM gene mutation of X.oryzae could reduce motility function of X.oryzae.
关键词
fkbMxoo/运动性/水稻白叶枯病菌Key words
fkbMXoo/Motility/Xanthomonas oryzae pv.oryzae引用本文复制引用
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据