摘要
[目的]研究重金属胁迫对草坪草种子萌发及幼苗生理特性的影响.[方法]以高羊茅和黑麦草为研究对象,采用水培方法培养高羊茅和黑麦草幼苗,研究了重金属Pb、Zn、Cu、Cd单一胁迫及Pb+Zn、Cu+Cd复合胁迫对草坪草种子萌发和幼苗生理特性的影响.[结果]Pb对高羊茅种子萌发、呼吸强度以及黑麦草种子电导率、呼吸强度的影响大于其余处理;Zn可缓解Pb胁迫对高羊茅种子发芽率、电导率和幼苗鲜重以及黑麦草种子萌发、电导率和幼苗鲜重的伤害;Pb+Zn复合胁迫对高羊茅种子电导率和幼苗叶绿素a(Chl a)、叶绿素 b(Chl b)、叶绿素a+b(Chl a+b)的影响有拮抗效应;Zn胁迫对黑麦草幼苗叶绿素a(Chl a)和类胡萝卜素(Car)的合成无显著影响;Cu+Cd复合胁迫对高羊茅种子萌发和幼苗鲜重的影响有协同效应,对黑麦草种子发芽率和幼苗叶绿素a(Chl a)、叶绿素b(Chl b)、叶绿素a+b(Chl a+b)的影响有拮抗效应.[结论]该研究结果可为今后重金属污染的治理提供理论依据.
Abstract
[Objective] To study the effects of heavy metals stress on the seed germination and seedling physiological characteristics of turfgrass.[Method] Taking Festuca arundinacea L.and Lolium perenne L.as research object, the effects of single stress of Pb, Zn, Cu, Cd, combined stress of Pb+Zn, Cu+Cd on the seed germination and seedling physiological characteristics of turfgrass were studied by using hydroponic culture method.[Result] Pb had stronger effects on the seed germination and respiratory intensity of F.arundinacea, the electrical conductivity and respiratory intensity of L.perenne than other treatments.Zn could alleviate the damage of Pb on seed germination percentage, electrical conductivity, seedling fresh weight of F.arundinacea, and the seed germination, electrical conductivity, seedling fresh weight of L.perenne.Pb+Zn combined stress had antagonistic effects on the seed electrical conductivity of F.arundinacea, Chl a, Chl b, Chl a+b in the seedlings of L.perenne.Zn stress had no significant effect on the synthesis of Chl a, Car in the seedlings of L.perenne.Cu+Cd combined stress had synergistic effects on seed germination and seedling fresh weight of F.arundinacea, but it had antagonistic effects on seed germination percentage and Chl a, Chl b, Chl a+b in the seedlings of L.perenne.[Conclusion] The research can provide theoretical basis for the treatment of heavy metals pollution in the future.
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