白芷种子蛋白质提取方法的比较
Comparison of Protein Extraction Methods for Angelica dahurica Seeds
吴萍 1郭俊霞 1李青苗 1夏燕莉 1张美 1肖特 1方清茂1
作者信息
- 1. 四川省中医药科学院,四川成都 610041
- 折叠
摘要
[目的] 建立适用于白芷种子的SDS-PAGE蛋白质样品提取方法.[方法]比较5种提取方法所获得白芷种子总蛋白质产量以及SDS-PAGE电泳的谱带数目、强度和电泳分辨率等方面的差异.[结果] 方法a所提蛋白质含量高,杂质少,电泳谱带清晰;方法b所提蛋白质溶液电泳谱带清晰,分辨率较高,但提取含量较低;方法c和d所提蛋白质杂质较多,纯度不高,分辨率较低;方法e所提蛋白质纯度较高,但蛋白条带缺失较多.[结论]方法a提取白芷种子蛋白质的效果最好,所提蛋白效率较高,杂质少,电泳谱带清晰,分辨率高,谱带数多,优于其他提取法.
Abstract
[Objective] To establish a suitable protein extraction protocol for total proteins extraction from Angelica dahurica seeds.[Method] We compared the total protein yield and SDS-PAGE electrophoretic band number and strength, lectrophoresis resolution by five different methods.[Result] Method a had higher protein content, less impurity and clearer electrophoresis band.Method b had clearer electrophoresis band, higher resolution, but lower protein content.Methods c and d had more impurity, lower purity and resolution.Method e had higher protein purity, but more lack of protein bands.[Conclusion] Method a has the best extraction effects of protein from A.dahurica seeds, which has higher protein extraction efficiency, less impurity, clearer band, higher resolution, more band number.Thus, method a is superior to other four methods.
关键词
白芷种子/蛋白质提取/聚丙烯酰氨凝胶电泳Key words
Angelica dahurica seed/Protein extraction/Polyacrylamide gel electrophoresis引用本文复制引用
基金项目
四川省基本科研业务专项(A2015N13)
四川省科技创新苗子工程(2016077)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据