基于ISSR标记的20种荷花品种资源遗传多样性分析
Genetic Diversity of 20 Nelumbo nucifera Gaertn.Varieties Revealed by ISSR Analysis
何天友 1沈少炎 1瞿印权 2陈凌艳 1刘颖嘉 1荣俊冬 2郑郁善3
作者信息
- 1. 福建农林大学园林学院,福建福州 350002
- 2. 福建农林大学林学院,福建福州 350002
- 3. 福建农林大学园林学院,福建福州 350002;福建农林大学林学院,福建福州 350002
- 折叠
摘要
[目的]研究20种荷花材料遗传多样性和亲缘关系.[方法]利用ISSR-PCR体系筛选出16条多态性引物,并利用16条ISSR引物对20种荷花材料进行PCR扩增.[结果]16条引物扩增出225个位点,多态性位点占75.56%.通过Popgene32软件计算出20个荷花品种间的相似性系数为0.577 8~0.951 1,平均值为0.779 6.通过聚类分析将20个荷花品种分为两大类,白洋淀红莲和冬瓜莲可归为一类,其他18个品种划分为第二类.[结论]ISSR标记技术可有效应用于不同荷花品种的遗传多样性分析和亲缘关系的鉴定.
Abstract
[Objective]To study the genetic diversity of 20 species of plant samples in Nelumbo nucifera Gaertn..[Method]16 primers were screened by using ISSR amplification system,and the DNA was used to amplify of 20 species of plant samples with 16 primers.[Result]16 primers produced 225 loci, the percentage of polymorphic loci was up to 75.56%.The genetic similarity coefficient of 20 species in squash ranged from 0.577 8 to 0.951 1,which was calculated by Popgene32.20 varieties by UPMGA analysis could be clustered into two groups,the first group included Baiyangdianhonglian and Donggualian;the rest of varieties was included in second group.[Conclusion]ISSR molecular markers could be effectively used in genetic diversity and fingerprint analysis for 20 species of plant samples in Nelumbo nucifera Gaertn..
关键词
荷花/ISSR/聚类分析/遗传多样性Key words
Nelumbo nucifera Gaertn./ISSR/Cluster analysis/Genetic diversity引用本文复制引用
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据