液相色谱-同位素质谱法鉴定蜂蜜掺假
Identification of Honey Adulteration by Liquid Chromatography-isotope Mass Spectrometry (LC-IRMS)
陈亚威 1张业鹏 1范志勇2
作者信息
- 1. 湖北工业大学机械工程学院,湖北武汉 430068;湖北省现代制造质量工程重点实验室,湖北武汉 430068
- 2. 湖北省现代制造质量工程重点实验室,湖北武汉 430068;湖北省食品质量安全监督检验研究院,湖北武汉 430068
- 折叠
摘要
[目的]探讨液相色谱-同位素质谱法(LC-IRMS)技术在蜂蜜掺假鉴定中的应用效果.[方法]采用LC-IRMS法检测随机抽样的62个蜂蜜样品,对蜂蜜中果糖(Fru)、葡萄糖(Glu)、二糖和三糖δ13C值进行测定.[结果]由于真实蜂蜜中果糖、葡萄糖、二糖和三糖的δ13C的最大值与最小值的差值小于2.1‰,果糖、葡萄糖的δ13C差值(δ13CFru-δ13CGlu)为-1‰ ~1‰,且不得含有寡糖,所以该次检测样品中有48.38%的蜂蜜不合格,掺假掺杂以添加C-3 植物源转化产物和淀粉糖浆为主,应加强对这类掺假的监控管理.[结论] LC-IRMS实现了蜂蜜中各种糖组分如果糖、葡萄糖、二糖和三糖等δ13C的分离与分析,可进行添加了C-3糖浆的掺假鉴定,大大提高了蜂蜜掺假的检测能力.
Abstract
[Objective] The aim was to explore application of LC-IRMS technique in identification of honey adulteration.[Method] We detected 62 honey samples by LC-IRMS, and determined δ13C value of fructose, glucose, disaccharide and trisaccharide of honey.[Result] The difference of maximum and minimum δ13C of fructose, glucose, disaccharide and trisaccharide of true honey was less than 2.1‰, and δ13CFru-δ13CGlu was from-1‰ to 1‰, and there was no oligosaccharides, so 48.38% of samples was no qualified.The adulteration was mainly C-3 plant products and starch syrup, so the monitoring management of adulteration should be strengthened.[Conclusion] LC-IRMS realizes the δ13C isolation and analysis of fructose, glucose, disaccharide and trisaccharide of honey, so it can be used in the adulteration identification of C-3 syrup and can improve the detection capability of honey adulteration.
关键词
液相色谱-同位素质谱/蜂蜜/掺假/δ13C值Key words
Liquid chromatography-isotope mass spectrometry (LC-IRMS)/Honey/Adulteration/δ13C value引用本文复制引用
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据