L-谷氨酰胺对麻疯树再生不定芽生根效果的影响
Effect of L-Glutamine on the Rooting of Regenerated Shoot Buds of Jatropha curcas
刘颖 1唐家年 1黄川腾 2刘国选 1林诗婉 1高美芳 1吴东霖 1陈建平3
作者信息
- 1. 广东海洋大学农学院,广东湛江 524088
- 2. 海南省林业科学研究所,海南海口 571100
- 3. 广东海洋大学食品科技学院,广东湛江 524088
- 折叠
摘要
[目的]建立促进麻疯树再生不定芽生根的组培体系.[方法] 通过在诱导麻疯树再生不定芽分化生根的培养基(MS+0.3 mg/L IBA)中添加一定浓度的L-谷氨酰胺(Gln)来达到促进芽条生根的效果.[结果] 在诱导麻疯树再生不定芽分化生根的培养基中添加20 mg/L Gln,可以显著提高芽条的生根率,生根率可达40%~50%.同时,可以在短时间内就获得较高的生根率,显著缩短了获得完整植株的周期.此外,还减少了生根过程中常见的再生植株叶片发黄、容易脱落现象的发生,显著提高了所获得的再生完整植株的质量.[结论] 麻疯树生根培养基的最优组合为添加0.3 mg/L IBA和20 mg/L Gln的MS培养基,应用该研究建立的组培体系可显著改善麻疯树再生不定芽的生根效果.
Abstract
[Objective] To establish a tissue culture system for promoting rooting of regenerated buds of Jatropha curcas.[Method] L-glutamine (Gln) was added into rooting medium (MS+0.3 mg/L IBA) for improving the rooting efficiency of regenerated buds of J.curcas.[Result]When 20 mg/L Gln was supplemented into rooting medium,the rooting efficiency of regenerated shoot buds could be significantly increased (raised up 40%-50%).In addition,satisfactory rooting rate was obtained in a short time (10~20 days),and the cycle of gaining a complete plant was shortened significantly.Moreover,reducing the chances of this happening that the leaves were easy to be yellowing and fall down from regenerated plants in the process of rooting,and then the quality of regenerated whole plants was obviously improved.[Conclusion] The optimal combination of rooting medium was MS medium supplemented with 0.3 mg/L IBA and 20 mg/L Gln.The rooting effect of regenerated buds of J.curcas could be significantly enhanced via application of the tissue culture system established in this study.
关键词
麻疯树/L-谷氨酰胺/再生不定芽/生根Key words
Jatropha curcas/L-glutamine/Regenerated shoot buds/Rooting引用本文复制引用
基金项目
湛江市科技攻关项目(2016B101)
大学生创新创业训练计划项目(CXXL2016015)
广东海洋大学博士科研启动项目()
广东海洋大学创新强校项目()
国家自然科学基金青年科学基金(21602034)
广东省自然科学基金博士启动项目(2016A30310332)
广东省天然产物绿色加工与产品安全重点实验室开放基金(201617)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据