Establishment of Near-infrared Quantitative Model for Protein in Oat and Its Application in the Breeding
[Objective] To study the feasibility of quantitatively analyzing protein content in oat by using near-infrared spectroscopy (NIRS) and discuss the changes of crude protein content in the same oat variety planted in different regions,to provide reference basis for improving the nutritional quality in the oat's breeding.[Method] 124 representative oat samples with a broad range of crude protein content were collected to make spectral scanning by using near-infrared grain quality analyzer.The contents of crude protein in samples were determined using traditional chemical method (GB/T 5009.5-2010).By the aid of near-infrared calibration software WinISI,the calibration model was established by using partial least square (PLS)method.The content of crude protein in 219 oat samples (17 varieties) was determined by using calibration model.The differences among different regions and different types were analyzed.[Result] The content of crude protein in 124 oat grain samples was 15.49%-23.77%,which had broader distribution range and good representativeness.The calibration model was successfully established with larger coefficient of determination and smaller standard error.The regression equation had a higher accuracy.Therefore,it was feasible and reliable to determine the content of crude protein in oat grains by using near-infrared spectroscopy technique.Near-infrared spectroscopy technique can replace chemical measurement method.[Conclusion]Near-infrared spectroscopy technique provides a new method for determining the content of crude protein in oat grains.
国家科技期刊平台
NSTL
万方数据
维普
