Study on the Optimization of Synthetic Culture Medium for Producing Arachidonic Acid with Mortierella alpina by Using Response Surface Methodology
[Objective] To study and optimize the ingredients of fermented liquid for synthetic culture medium for producing arachidonic acid with Mortierella alpine and increase the yield of ARA.[Method] The carbon sources in the culture medium were optimized,single carbon source and composite carbon source were screened and their initial concentrations were optimized.Many kinds of inorganic nitrogen sources were screened out by using single factor test.After steepest ascent test on four significant factors(glucose,glycerol,yeast powder and amino acid mixture)with significant effects,significant factors were optimized by using response surface center combination design.[Result] The optimum carbon source combination was 80 g/L glucose + 20 g/L glycerol.Taking yeast powder as nitrogen source,amino acid mixture added in the culture medium as a growth factor could promote the accumulation of ARA in the fermentation liquid of M.alpine.The optimized synthetic medium was composed of 80 g/L glucose,12 g/L glycerol,20 g/L yeast powder and 0.3 g/L amino acid mixture.The optimized synthetic medium was verified and the yield was detected after shake flask fermentation culture 7 days.The average yield of ARA was 7.084 1 g/L,which was close to the predicted value.[Conclusion] The research results can provide research basis for improving the yield of ARA in the industrial production of ARA further.
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维普
