高活性纳豆激酶工艺改进研究
Study on the Improvement of High Activity Natto Kinase
张浩 1王家林1
作者信息
- 1. 青岛科技大学生物工程实验室,山东青岛 266042
- 折叠
摘要
[目的]优化高活性纳豆激酶的纳豆生产工艺条件.[方法]从市售纳豆产品中分离筛选出高活性的菌株作为试验菌株,采用固态发酵的方法制备纳豆,对影响纳豆激酶产量的主要因素浸泡时间、接种量、发酵时间等进行考察,研究高活性纳豆激酶的工艺条件.[结果]利用对甲基苯磺酰L精氨酸甲酯(TAME)法测定纳豆激酶活性,确定最佳纳豆激酶酶活的纳豆生产工艺条件为浸泡时间8 h、接种量10%、发酵时间48 h.[结论]该研究可为高活性纳豆激酶的生产提供参考.
Abstract
[Objective] To optimize the process conditions of high activity natto kinase.[Method]Natto was isolated from commercial natto products and used as the experimental strain.Natto was prepared by solid-state fermentation.The process conditions of high activity natto kinase were studied.The main factors affecting the yield of natto kinase were as follows:immersion time,inoculation amount and fermentation time.[Result]The activity of natto kinase was determined by TAME method.The optimum natto kinase production process conditions were determined as 8 h soaking time,10% inoculation amount,48 h fermentation time.[Conclusion] The study can provide reference for production of high activity natto kinase.
关键词
纳豆/纳豆激酶/活性测定/工艺优化Key words
Natto/Natto kinase/Activity determination/Process optimization引用本文复制引用
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据