摘要
[目的]优化红龙草色素提取工艺,并研究其稳定性.[方法]以红龙草全草为材料,通过单因素试验和正交试验优化红龙草色素提取工艺,并对提取的红龙草色素稳定性进行研究.[结果]红龙草色素的最佳提取条件:超声时间30 min,料液比1∶55(g∶mL),α-淀粉酶∶纤维素酶为3∶1,即在溶液中按比例加入0.2%纤维素酶和0.6%α-淀粉酶.光照、pH、过氧化氢、亚硫酸钠、苯甲酸、山梨酸、金属离子Zn2+、Cu2+、Ca2+、Fe3+对色素稳定性具有一定的影响.[结论]该研究可为红龙草色素的开发利用提供参考.
Abstract
[Objective] To optimize the extraction process of Altemanthera Ficoidea cv.Ruliginosa pigment and study its stability.[Method] By single-factor and L9(34)orthogonal experiment, extraction conditions of pigment from Altemanthera Ficoidea cv.Ruliginosa were optimized, and the effects on the stability of pigment were investigated.[Result] The results showed that the optimum technological conditions of the extraction of pigment from Altemanthera Ficoidea cv.Ruliginosa were distilled water for extraction agents, 30 min for extraction time, 1∶55(g∶mL) for the ratio of the solid and liquid, 1∶3 for the ratio of cellulose to alpha amylase, namely adding 0.2% cellulose and 0.6% alpha amylase into solution.Light, pH, H2O2 , sulfurous acid, benzoic acid, sorbic acid, metal ions including Zn2+, Cu2+, Ca2+ and Fe3+ had effects on the stability of pigment.[Conclusion] The study can provide reference for development and utilization of Altemanthera Ficoidea cv.Ruliginosa pigment.