H9N2亚型禽流感病毒单克隆抗体的制备及其表位鉴定
Preparation of H9N2 Subtype Avian Influenza Virus Antibodies and Epitope Identification
刘晓婧 1凌红丽 1蒋贻海 1张园园 1赵明 1杨光烈 1于春梅 1薄宗义1
作者信息
- 1. 青岛蔚蓝生物制品有限公司,山东青岛 266114
- 折叠
摘要
[目的]制备H9N2亚型禽流感病毒单克隆抗体和鉴定表位.[方法]选取H9N2亚型禽流感病毒(AIV)WD-1株的纯化抗原免疫BALB/c小鼠,对获得2株抗H9N2亚型禽流感病毒的特异性单抗4C10和6E3表位进行鉴定.[结果]单抗4C10和6E3分别属于IgG1和IgG2b亚型,ELISA检测效价分别为1∶103和1∶105;HI效价分别为215和214;2株单抗均具有鸡胚中和活性.利用噬菌体展示表位技术对2株单抗的抗原表位进行鉴定,结果显示均为针对HA 蛋白的1个线性表位.[结论]该研究为禽流感病毒快速诊断方法的建立提供了依据.
Abstract
[Objective] To prepare H9N2 subtype avian influenza virus antibodies and identify epitope.[Method] The epitopes of two monoclonal antibodies (McAbs) specific to H9 subtype avian influenza virus (AIV) were identified by cell fusion with the BALB/c mice immunized with purified H9N2 AIV WD-1.[Result]The 4C10 and 6E3 were belonged to IgG 1 and IgG 2b,repectively.The ELISA titers were 1∶103 and 1∶105.The HI titers in their ascites were 215 and 214.Two monoclonal antibodies had chicken embryo neutralizing activity.The antigen epitopes of two monoclonal antibodies were all linera epitopes targeting HA protein.[Conclusion] The study laid the foundation for the establishment of rapid diagnostic method of avian influenza virus.
关键词
禽流感病毒/H9N2亚型/单抗/抗原表位Key words
Avian influenza virus/H9N2 subtype/Monoclonal antibody/Antigen epitopes引用本文复制引用
基金项目
国家国际科技合作专项(2014DFA31830)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据